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Image Search Results
Journal: Cell Death & Disease
Article Title: Circular RNA hsa_circ_0006401 promotes proliferation and metastasis in colorectal carcinoma
doi: 10.1038/s41419-021-03714-8
Figure Lengend Snippet: A Schematic representation of plasmid construction. B Detection of GFP fluorescence. The indicated constructs were transfected into HeLa cells for 24 h. C Western blot analysis with anti-hsa_circ_0006401 antibodies to evaluate proteins from SW620 cells transfected with different constructs. NC (negative control), p-Circ (hsa_circ_0006401 circRNA level was highly expressed), ORFmut (ATG start codon of p-Circ was mutated to TTG), siRNA (hsa_circ_0006401 circRNA level was silenced by siRNA). GAPDH was used as a loading control. D qRT-PCR analysis of hsa_circ_0006401 cirRNA expression level of different groups. E qRT-PCR analysis of hsa_circ_0006401 cirRNA expression level of different groups of SW480 cells. NC (negative control), col6a3 (col6a3 overexpression), p-Circ (hsa_circ_0006401 circRNA level was highly expressed), ORFmut (ATG start codon of p-Circ was mutated to TTG). F Western blot analysis with anti-hsa_circ_0006401 antibodies (HAPL0559) to evaluate proteins from SW480 cells transfected with different constructs. NC (negative control), col6a3 (col6a3 overexpression), p-Circ (hsa_circ_0006401 circRNA level was highly expressed), ORFmut (ATG start codon of p-Circ was mutated to TTG). G Reprehensive images of IHC analysis with an antibody (HAPM0617) to evaluate proteins from colon cancer tissues and normal colon tissues (left panel). IHC scores were calculated (right panel). Error bars indicate SD, * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Proteins were isolated from CRC cells and incubated with primary antibody detecting hsa_circ_0006401 peptide (1:1000 dilution, HuaAn Biotechnology Co., Ltd),
Techniques: Plasmid Preparation, Fluorescence, Construct, Transfection, Western Blot, Negative Control, Quantitative RT-PCR, Expressing, Over Expression
Journal: Cell Death & Disease
Article Title: Circular RNA hsa_circ_0006401 promotes proliferation and metastasis in colorectal carcinoma
doi: 10.1038/s41419-021-03714-8
Figure Lengend Snippet: A Gene set analysis of COL6A3 expression in normal tissue and colon cancer using TCGA cancer browser. The number of patients from each subtype is indicated below the box plot. B COL6A3 expression was assessed by Kaplan–Meier survival analysis for 5-year overall survival outcome in 270 colon cancer patients. C The correlation of hsa_circ_0006401 and COL6A3 mRNA in 12 human CRC tissues. * p < 0.05. D COL6A3 mRNA expression level in negative control SW620 cancer cells (NC) and hsa_circ_0006401 silencing SW620 cancer cells (siRNA). ** p < 0.01. E COL6A3 protein expression level in SW620 cells transfected with different constructs. NC (negative control), p-Circ (hsa_circ_0006401 circRNA level was highly expressed), ORFmut (ATG start codon of p-Circ was mutated to TTG), siRNA (hsa_circ_0006401 circRNA level was silenced by siRNA). F TGFβ1 mRNA expression level in negative control SW620 cancer cells (NC) and hsa_circ_0006401 silencing SW620 cancer cells (siRNA). * p < 0.05. Error bars indicate SD.
Article Snippet: Proteins were isolated from CRC cells and incubated with primary antibody detecting hsa_circ_0006401 peptide (1:1000 dilution, HuaAn Biotechnology Co., Ltd),
Techniques: Expressing, Negative Control, Transfection, Construct
Journal: Cell Death & Disease
Article Title: Circular RNA hsa_circ_0006401 promotes proliferation and metastasis in colorectal carcinoma
doi: 10.1038/s41419-021-03714-8
Figure Lengend Snippet: A HA tag expression in negative control SW480 cancer cells (NC) and hsa_circ_0006401 ORF-198aa-HA tag plasmid transfected SW480 cancer cells (ORF-198aa-HA). B Representative images of the location of hsa_circ_0006401 peptide in SW480 cells. C Gene ontology (GO) analysis for hsa_circ_0006401 peptide combined proteins. BP represents biological processes, CC represents cellular components and MF represents molecular functions. D Decay of col6a3 and GAPDH mRNA was monitored in SW480 cells treated with Actinomycin D with indicated time points. NC (negative control), siRNA (hsa_circ_0006401 circRNA level was silenced by siRNA). Min represents minutes. Error bars indicate SD, and data were fitted by linear regression. * p < 0.05.
Article Snippet: Proteins were isolated from CRC cells and incubated with primary antibody detecting hsa_circ_0006401 peptide (1:1000 dilution, HuaAn Biotechnology Co., Ltd),
Techniques: Expressing, Negative Control, Plasmid Preparation, Transfection